Effects of second order photobleaching on recovered diffusion parameters from fluorescence photobleaching recovery
نویسندگان
چکیده
منابع مشابه
Fluorescence recovery after photobleaching: the case of anomalous diffusion.
The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations. We propose an approach based on the CTRW and the corresponding fractional d...
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The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods. Fluorescence recovery after photobleaching (FRAP) experiments ...
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Multi-fluorescence recovery after photobleaching is a microscopy technique used to measure the diffusion coefficient (or analogous transport parameters) of macromolecules, and can be applied to both in vitro and in vivo biological systems. Multi-fluorescence recovery after photobleaching is performed by photobleaching a region of interest within a fluorescent sample using an intense laser flash...
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The autofluorescence of the retina that originates primarily from lipofuscin fluorophores in retinal pigment epithelial cells, is observed to undergo photobleaching during the acquisition of fundus autofluorescence images. Bisretinoid fluorophores isolated from retinal pigment epithelial cells have the spectral characteristics consistent with their being the source of fundus autofluorescence. C...
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Fluorescence recovery after photobleaching (FRAP) is used to determine diffusion constants for the dye molecule 6-propionyl-2-dimethylaminonaphthalene (prodan) on silica and on a variety of organic monolayers grafted onto silica. Similar diffusion constants of ∼2 × 10-7 cm2/s are found for prodan on chlorotrimethylsilane (CTS) based self-assembled monolayers and on silanated poly(ethylene glyco...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1991
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(91)82148-8